mouse anti-map2 monoclonal ab  (Millipore)

Journal: PLoS ONE

Article Title: Cell Surface Estrogen Receptor Alpha Is Upregulated during Subchronic Metabolic Stress and Inhibits Neuronal Cell Degeneration

doi: 10.1371/journal.pone.0042339

Figure Lengend Snippet: mERα expression (green fluorescence) in unpermeated SH-SY5Y cells and MAP2 expression (red fluorescence) in Triton X100 permeated cells: ( A ) untreated, ( B ) CMP or ( C ) NAC/CMP treated cells. Cells were counterstained with Hoechst dye to reveal nuclei (blue staining). Note the yellow spots suggesting a colocalization of ERα and MAP2 in CMP exposed cells. No yellow spots are instead observable in untreated and NAC/CMP treated cells. Magnification: 3500×.

Article Snippet: After washing three times with PBS, cells were incubated for 1 h at 4°C with rabbit anti-human ERα MC-20 polyclonal Ab (Santa Cruz), mouse anti-human ERβ 1531 monoclonal Ab (Santa Cruz), and mouse anti-MAP2 monoclonal Ab (Sigma).

Techniques: Expressing, Fluorescence, Staining

Journal: PLoS ONE

Article Title: Cell Surface Estrogen Receptor Alpha Is Upregulated during Subchronic Metabolic Stress and Inhibits Neuronal Cell Degeneration

doi: 10.1371/journal.pone.0042339

Figure Lengend Snippet: mERα expression (green fluorescence) in unpermeated hippocampal neuronal cells and MAP2 expression (red fluorescence) in Triton X100 permeated hippocampal neuronal cells: ( A ) untreated, ( B, C ) CMP treated or ( D ) NAC exposed before CMP treatment. Note the yellow spots in ( B ) and ( C ) suggesting a colocalization of ERα and MAP2 in soma ( B ) and dendritic protrusions ( C ) of CMP exposed cells. No yellow spots are instead observable in untreated and NAC/CMP exposed hippocampal cells. Cells were counterstained with Hoechst dye to reveal nuclei (blue staining). Magnifications: ( A ) ( B ) ( D ) 3500×; ( C ) 6000×.

Article Snippet: After washing three times with PBS, cells were incubated for 1 h at 4°C with rabbit anti-human ERα MC-20 polyclonal Ab (Santa Cruz), mouse anti-human ERβ 1531 monoclonal Ab (Santa Cruz), and mouse anti-MAP2 monoclonal Ab (Sigma).

Techniques: Expressing, Fluorescence, Staining

Journal: PLoS ONE

Article Title: Avian Adeno-Associated Virus Vector Efficiently Transduces Neurons in the Embryonic and Post-Embryonic Chicken Brain

doi: 10.1371/journal.pone.0048730

Figure Lengend Snippet: (A) Primary cultures of chick neural cells after A3V or LV infection were immuno-labeled with antibodies against EGFP (green) and the neuronal marker MAP2 (red). Arrowheads indicate MAP2-negative, LV-transduced cells. Scale bar indicates 20 µm. (B) The neuronal transduction rates of A3V and LV are represented as the percentage of MAP2 and EGFP double-positive cells within 100–200 EGFP-positive cells (n = 4). *p<0.005. The raw data are listed in .

Article Snippet: The primary antibodies (Abs) used were polyclonal rabbit anti-EGFP Ab (1∶1000; Life Technologies) and monoclonal mouse anti-microtubule-associated protein 2 (MAP2) Ab (1∶1000; Millipore, Bedford, MA, USA).

Techniques: Infection, Labeling, Marker, Transduction

Journal: PLoS ONE

Article Title: Avian Adeno-Associated Virus Vector Efficiently Transduces Neurons in the Embryonic and Post-Embryonic Chicken Brain

doi: 10.1371/journal.pone.0048730

Figure Lengend Snippet: (A) Tet inducible A3V constructs: rtTAV16, reverse tetracycline-controlled transactivator variant 16; TRE, tetracycline response element. (B) A3V-RSV-rtTAV16 and A3V-TRE-EGFP (0.5–1.5 µl, 1×10 13 GC/ml each) were injected at E3.0, and Dox was administered at E6.5. Immunofluorescence labeling was conducted at E20 (n = 2 embryos) or E20.5 (n = 2 embryos). (C) Anti-EGFP immunofluorescence on a coronal section of the NL-NM circuits at E20.5. The EGFP signal was not detected in the cell bodies of the majority of NL neurons, but strong EGFP signal was observed in the cell bodies of some NL neurons (arrowheads). Scale bar indicates 100 µm. (D) A magnified view of NL neurons at E20.5, visualized with double immunofluorescence labeling for EGFP (green) and dendrite marker MAP2 (red). The immunofluorescence of EGFP was clearly separated from that of MAP2 in the NL dendritic portion. Scale bar indicates 20 µm.

Article Snippet: The primary antibodies (Abs) used were polyclonal rabbit anti-EGFP Ab (1∶1000; Life Technologies) and monoclonal mouse anti-microtubule-associated protein 2 (MAP2) Ab (1∶1000; Millipore, Bedford, MA, USA).

Techniques: Construct, Variant Assay, Injection, Immunofluorescence, Labeling, Marker